"Chamber method - This method employs a top-opening chamber into which the animal(s) are introduced . After the animal(s) are placed in the chamber, a slow flow of CO2 is intiated for a few minutes to slowly establish a high concentration at the bottom of the chamber. It is an unacceptable practice to pre-charge the chamber with CO2 prior to placing the animals in the chamber."
[url]http://research.uiowa.edu/animal/?get=euthanasia#Carbon dioxide[/url]
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